Ludmila Kousoulidou

Academic Staff

Current Position

Associate Scientist, Department of Cytogenetics and Genomics  

The Cyprus Institute of Neurology & Genetics


1. BSc. degree, (Biology), 1989-1994, Lomonosov Moscow State University, Genetics and Selection department. 
2. MSc. Degree, (Genetics and selection), 1994-1995, Lomonosov Moscow State University, Genetics and Selection department. 
3. PhD degree, (Genetics), 1996-2001, Lomonosov Moscow State University, Genetics and Selection department. Thesis in collaboration with Engelhard Institute of Molecular Genetics of the Russian Academy of Sciences.

Research Interests

Grants,  Principal Investigator/Coordinator: 
  • 2008-2010, Cyprus Research Promotion Foundation; ΥΓΕΙΑ/ΔΥΓΕΙΑ/0308(ΒΙΕ)/18, High-resolution genetic investigation of autism in Cyprus. 
  • 2009-2011, Telethon Grant; Targeted screening of microRNA – coding genes in patients with Autism: 100,000 €. 
Grants, Research team member/co-investigator:
  • 2007-2010, Cyprus Research Promotion Foundation; EPYEΞ/0406/09; Genetic Investigation of Syndromes with X chromosome anomalies.. 
  • 2007-2010, Cyprus Research Promotion Foundation; EPYEΞ/0506/29; “SYNDROMA”; Genetic investigation and characterization of unknown genetic and neurological syndromes in the population of Cyprus, using high-resolution DNA microarrays.
  • 2002-2006, 5th European Union Framework Program, European Union; The Genetic and Neurobiological Basis of X-Linked Mental Retardation (EURO-MRX).
  • 2008-2012, 7th EU Framework, European Union; FP7-HEALTH-2007-2.2.1-10-223692; Improving Diagnoses of Mental Retardation in Children in Eastern Europe and Central Asia through Genetic Characterisation and Bioinformatics/-Statistics (CHERISH).
  • 2004-2009,  6th EU Framework, European Union; LSHB-CT-2004-503243; Special Non-Invasive Advances in Fetal and Neonatal Evaluation Network (SAFE). 
  • 2009-2012, 7th European Union Framework Program, European Union; Point of care monitoring and diagnostics for autoimmune diseases, (POCEMON). 


Dr. Ludmila Kousoulidou was employed at the Department of Cytogenetics and Genomics of the Cyprus Institute of Neurology and Genetics (CING) in 2001 and until present holds the position of post-doctoral researcher. She is a member of the European Society of Human Genetics and the Cyprus Society of Human Genetics.  


Selected Publications:

  1. Kousoulidou LK, Karpova NN, Razorenova OV, Glukhov IA, Kim AI, Lyubomirskaya NV, Il’in IV. MDG4 (gypsy) retrotransposon polymorphism in Drosophila melanogasret strains. Doklady Academii Nauk (Proceedings of the Academy of Science). 2001 Jan; 376(1): 133-138. Russian.
  2. Patsalis PC, Skordis N, Sismani C, Kousoulidou L, Koumbaris G, Eftychi C, Stavrides G, Ioulianos A, Kitsiou-Tzeli S, Galla-Voumvouraki A, Kosmaidou Z, Hadjiathanasiou CG, McElreavey K. Identification of high frequency of Y chromosome deletions in patients with sex chromosome mosaicism and correlation with the clinical phenotype and Y-chromosome instability. Am J Med Genet A. 2005 Jun 1;135(2):145-9.
  3. Patsalis PC, Kousoulidou L, Sismani C, Mannik K, Kurg. MAPH: from gels to microarrays. Eur J Med Genet. 2005 Jul-Sep;48(3):241-9.
  4. Kousoulidou L., Parkel S., Zilina O., Palta P., Puusepp H., Remm M. , Gecz J., Turner G., Boyle J., van Bokhoven H., de Brouwer A. , Van Esch H., Froyen G. , Ropers H ., Chelly J ., Moraine C ., Kurg A. , Patsalis P.C. Screening of 20 patients from XLMR families for X-chromosomal subtle copy number alterations, using chromosome X–specific array-MAPH platform. Eur J Med Genet, 2007 Nov-Dec;50(6):399-410.
  5. Kousoulidou L , et al. . Array-MAPH: a methodology for the detection of locus copy-number changes in complex genomes. Nat Protoc 2008;3(5):849-65.
  6. Bashiardes S.,Kousoulidou L., van Bokhoven H., Ropers H. , Chelly J., Moraine C, de Brouwer APM., Van Esch H., Froyen G. and Patsalis P.C. A new chromosome X exon-specific microarray platform for screening of patients with Xlinked disorders. J Mol Diagn November 2009 Vol. 11, No 6.
  7. Kousoulidou L, Sismani C, Patsalis PC. Multiplex Amplifiable Probe Hybridization (MAPH) methodology as an alternative to comparative genomic hybridization (CGH). Methods Mol Biol. 2010;653:47-71.
  8. Kousoulidou L, Tanteles G,  Moutafi M, Sismani C, Patsalis PC,  Anastasiadou V. 263.4 kb deletion within the TCF4 gene consistent with Pitt-Hopkins syndrome, inherited from a mosaic parent with normal phenotype. Eur J Med Genet. 2013 Mar 23. pii: S1769-7212(13)00065-7. doi: 10.1016/j.ejmg.2013.03.005.
  9. Kousoulidou L, et al., “Screening of 50 Cypriot Patients with Autism Spectrum Disorders or Autistic Features Using 400K Custom Array-CGH,” BioMed Research International, vol. 2013, Article ID 843027, 5 pages, 2013. doi:10.1155/2013/843027
  10. Koufaris C, Papagregoriou G, Kousoulidou L, Moutafi M, Tauber M, Jouret B, Kieffer I, Deltas C, Tanteles GA, Anastasiadou V, Patsalis PC, Sismani C. “Haploinsufficiency of the miR-873/miR-876 microRNA cluster is associated with craniofacial abnormalities” Gene. 2015 Apr 25;561(1):95-100. doi:10.1016/j.gene.2015.02.018. Epub 2015 Feb 1
  11. Angelos Alexandrou, Ioannis Papaevripidou, Ioanna Maria Alexandrou, Athina Theodosiou, Paola Evangelidou, Ludmila Kousoulidou, George Tanteles, Violetta Christophidou‐Anastasiadou, Carolina Sismani. De novo mosaic MECP2 mutation in a female with Rett syndrome. Clin Case Rep. 2019;1–5.
  12. Kousoulidou L, Alexandrou A, Papaevripidou I, Evangelidou P, Tanteles G, Anastasiadou VC, Sismani C. Two unrelated individuals carrying rare mosaic deletions in TCF4 gene. Am J Med Genet A. 2018 Nov 18. doi: 10.1002/ajmg.a.60692


  1. First Choremeio Award at the 42nd Pan-Hellenic Paediatric Meeting, 2004. Y chromosome deletions in children with sex chromosome mosaicism. Possible pathogenic mechanism of sex chromosome aneuploidy development. Team member. 
  2. Best poster award at 7th Course in Molecular Cytogenetics and DNA Microarrays, November 2005, Bertinoro, Italy. Array-MAPH: A novel microarray technology for genomic analysis. Principal investigator. Presenting author Olga Zilina.  
  3. Nicos Symeonides research Award, 2008, Cyprus Research Promotion Foundation. The genetic basis of X-linked Mental retardation. Post-doctoral researcher. 
  4. National Award for Innovation, 2009, Research Promotion Foundation, received in June 2010. Development of a DNA microarray which enables the detection of unknown X-linked disorders. Team member.
winner aashe copernicus